Sonic Hedgehog antibody

Catalog No. ABIN6147930

The Rabbit Monoclonal anti-Sonic Hedgehog antibody has been validated for WB and IF. It is suitable to detect Sonic Hedgehog in samples from Human.

Quick Overview for Sonic Hedgehog antibody (ABIN6147930)

Target: Sonic Hedgehog (SHH)

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Sonic Hedgehog antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-Sonic Hedgehog Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Monoclonal Antibodies

Purification: Affinity purification

Immunogen: A synthesized peptide derived from human Sonic Hedgehog (Shh) (Shh)

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000, IF,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,0.05 % BSA,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Alternative Name: SHH

Background: This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE). It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly.,HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS,Angiogenesis,Cancer,Cardiovascular,Cell Biology & Developmental Biology,Embryonic Stem Cells,Endocrine & Metabolism,Epigenetics & Nuclear Signaling,Hedgehog Signaling Pathway,Neural Stem Cells,Stem Cells,Tumor suppressors,SHH

Molecular Weight: 51 kDa

Gene ID: 6469

UniProt: Q15465

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development